Complete Evaluation of the Antigenic Affect of Human Papillomavirus Lineage Variation on Recognition by Neutralizing Monoclonal Antibodies Raised Towards Lineage A Main Capsid Proteins of Vaccine-Associated Genotypes
Human papillomavirus (HPV) is the causative agent of cervical and different epithelial cancers. Naturally occurring variants of HPV have been categorised into lineages and sublineages primarily based upon their complete genome sequence, however little is understood in regards to the impression of this variety on the construction and performance of viral gene merchandise. The HPV capsid is an icosahedral lattice comprising 72 pentamers of the most important capsid protein (L1) and the related minor capsid protein (L2).
We investigated the potential impression of this genome variation on the capsid antigenicity of lineage and sublineage variants of seven vaccine-relevant, oncogenic HPV genotypes by utilizing a big panel of monoclonal antibodies (MAbs) raised towards the L1 proteins of lineage A antigens.
Every genotype had not less than one variant that displayed a ≥4-fold lowered neutralizing antibody sensitivity towards not less than one MAb, demonstrating that naturally occurring variation can have an effect on a number of useful antigenic determinants on the HPV capsid. For HPV16, HPV18, HPV31 and HPV45, the general impression was of a low magnitude.
For HPV33 (sublineages A2 and A3 and lineages B and C), HPV52 (lineage D) and HPV58 (lineage C), nevertheless, variant residues within the indicated lineage and sublineages lowered their sensitivity to neutralization by all MAbs by as much as 1000-fold, suggesting the presence of key antigenic determinants on the floor of those capsids. These determinants had been resolved additional by site-directed mutagenesis. These knowledge enhance our understanding of the impression of naturally occurring variation on the antigenicity of the HPV capsid of vaccine-relevant oncogenic HPV genotypes.
IMPORTANCEHuman papillomavirus (HPV) is the causative agent of cervical and another epithelial cancers. HPV vaccines generate useful (neutralizing) antibodies that concentrate on the virus particles (or capsids) of the commonest HPV cancer-causing genotypes.
Every genotype contains variant varieties which have arisen over millennia and which embody adjustments inside the capsid proteins. On this research, we explored the potential for these naturally occurring variant capsids to impression recognition by neutralizing monoclonal antibodies.
All genotypes included not less than one variant kind that exhibited lowered recognition by not less than one antibody, with some genotypes affected greater than others. These knowledge spotlight the impression of naturally occurring variation on the construction of the HPV capsid proteins of vaccine-relevant oncogenic HPV genotypes.
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Rabbit Polyclonal antibody against PDGFR-? from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA
Description: A Monoclonal antibody against Human PDGFRB / PDGFR Beta (clone 42G12). The antibodies are raised in Mouse and are from clone 42G12. This antibody is applicable in WB and IHC-P
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Rat. This PDGFR-Beta antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y771
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Rat. This PDGFR-Beta antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y771
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Rat. This PDGFR-Beta antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y771
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGFR-?
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGFR-?
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PDGFR-?
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y754
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y754
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y754
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y849
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y849
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y849
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1018
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1018
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1018
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y762
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y762
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y762
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1009
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1009
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1009
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1021
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1021
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y1021
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y740
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y740
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y740
Description: A polyclonal antibody for detection of PDGFR Alpha from Human, Mouse, Rat. This PDGFR Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDGFR ? protein
Description: A polyclonal antibody for detection of PDGFR Alpha from Human, Mouse, Rat. This PDGFR Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDGFR ? protein
Description: A polyclonal antibody for detection of PDGFR Alpha from Human, Mouse, Rat. This PDGFR Alpha antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PDGFR ? protein
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human PDGFR-? at AA range: 1010-1090
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human PDGFR-? at AA range: 1010-1090
Description: A polyclonal antibody for detection of PDGFR-Alpha from Human, Mouse, Rat. This PDGFR-Alpha antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human PDGFR-? at AA range: 1010-1090
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y751
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y751
Description: A polyclonal antibody for detection of PDGFR-Beta from Human, Mouse, Rat. This PDGFR-Beta antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human PDGFR-? around the non-phosphorylation site of Y751
Monoclonal antibody stability might be usefully monitored utilizing the excitation-energy-dependent fluorescence edge-shift
Among the many main challenges within the improvement of biopharmaceuticals are structural heterogeneity and aggregation. The event of a profitable therapeutic monoclonal antibody (mAb) requires each a extremely lively and likewise secure molecule.
While a spread of experimental (biophysical) approaches exist to trace adjustments in stability of proteins, routine prediction of stability stays difficult. The fluorescence purple edge excitation shift (REES) phenomenon is delicate to a spread of adjustments in protein construction. Primarily based on current work, we’ve discovered that quantifying the REES impact is extraordinarily delicate to adjustments in protein conformational state and dynamics.
Given the acute sensitivity, doubtlessly this software may present a ‘fingerprint’ of the construction and stability of a protein, which might have functions within the discovery and improvement of biopharamceuticals. As such we’ve explored our speculation with a panel of therapeutic mAbs. We reveal that the quantified REES knowledge present outstanding sensitivity, having the ability to discern between structurally an identical antibodies and displaying sensitivity to unfolding and aggregation.
The method works throughout a broad focus vary (μg -mg/ml) and is extremely constant. We present that the method might be utilized alongside conventional characterisation testing inside the context of a compelled degradation research (FDS). We reveal the method is ready to predict the steadiness of mAbs each within the brief (hours), medium (days) and long-term (months). The method advantages from low technical complexity, is speedy and makes use of instrumentation which exists in most biochemistry laboratories with out modification.
Description: A Monoclonal antibody against Human TXN (monoclonal) (M04). The antibodies are raised in mouse and are from clone 6C10. This antibody is applicable in WB and IF, E
Description: A Monoclonal antibody against Human STAT6 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 6C10. This antibody is applicable in WB, IHC and IF, E
Description: For the quantitative determination of swine tumor necrosis factor(TNF-α) concentrations in cell culture supernates, serum, and plasma.This package insert must be read in its entirety before using this product.
Description: The Mouse Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit is to be used for the vitro quantitative determination of Mouse TNF-α in serum, plasma, tissue lysates, cell culture supernatants and other biological fluids. The Kit is intended for research use only, not for diagnostic or therapeutic procedure. If detection of other special sample, please contact our technical support.
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
